血透患者透析前后外周血超氧化物岐化酶测定及临床意义

CLINICAL STUDY OF PLASMA SOD CHANGES IN VENOUS BLOOD BEFORE AND AFTER HEMODIALYSIS

目的 :通过测定尿毒症患者血液透析前后外周血超氧化物岐化酶 (SOD)的活性变化 ,探讨血液透析疗法对SOD活性的影响作用。方法 :用化学发光法测定SOD活性以比较血液透析前后SOD精浆含量。结果 :健康对照组与慢性肾功能不全病人血浆SOD浓度分别为 (2 85 .31± 6 8.41)u/ml与 (196 .5 3± 45 .42 )u/ml(P <0 .0 5 ) ,尿毒症病人血液透析前后血浆SOD浓度分别为 (196 .5 3± 45 .42 )u/ml与 (2 93 .76± 72 .6 1)u/ml(P <0 .0 5 )。结论 :血液透析法可激活体内氧自由基清除剂活性 ,消除体内过多氧自由基 。

Objective:To observe the changes in the plasma SOD activity before and after hemodialysis in order to investigate the latter's effet on SOD activity.Methods:Compare the different level of SOD in plasma before and after hemodialysis by testing its activity through chemiluminescence.Results:The plasma concentrations of SOD in healthy control group and chronic renal failure group were 285.31±68.41u/ml and 196.53±45.42u/ml respectively,the level of SOD in control group was remarkably higher than that in chronic renal failure group (P<0.05).The plasma levels of SOD in uremia before and after hemodialysis were 196.53±45.42u/ml and 293.76±72.61u/ml respectively.With hemodialysis,SOD concentration was elevated (P<0.05).Conclusions:Hemodialysis can impede the progress of renal failure by activating SOD to eliminate the excessive oxygen-derived free radicals in vitro.