摘要
目的 :克隆人白细胞介素 18(IL - 18)全长cDNA基因 ,进一步探讨人白细胞介素 18的功效。方法 :采用RT -PCR的方法从人胚肝组织中扩增出人白细胞介素 18(IL - 18)基因 ,并克隆到真核表达的绿色荧光蛋白报告载体pEGFP -N1中。结果 :经PCR及DNA序列测定得以证实其序列与文献报导一致。结论 :该实验成功地克隆了IL - 18cDNA ,并将其重组构建了含有IL - 18cDNA的真核表达载体pEGFP -N1-IL18。
Objective:In order to clone the whole cDNA fragment of human interleukin 18(hIL-8),and to find its furthe efficacy.Methods:Human interleukin-18 (hIL-18) was amplified from human embryo liver tissue by Reverse Transcription-Polymerase Chain Reaction (RT-PCR).Then it was subcloned into an expression vector in eukaryote-pEGFP-N1 whose report gene can express green flouorescence protein.Results:It was defined by PCR and DNA sequencing.Conclusions:IL-18 cDNA was cloned successfully,and it was subcloned into an vector to from a new eukaryote expression vector-pEGFP-N1-IL18.
出处
《中国现代医学杂志》
CAS
CSCD
2001年第4期30-32,共3页
China Journal of Modern Medicine