舌癌中Langerhans细胞的图像分析研究

QUANTITATIVE ANALYSIS OF ATP-ASE POSITIVE LANGERHANS CELL IN HUMAN TONGUE SQUAMOUS CELL CARCINOMA

目的 :探讨Langerhans细胞学与舌癌发生、发展的关系。方法 :采用电子计算机图像分析方法对 30例人类舌癌标本中正常上皮、不典型增生、原位癌及浸润癌ATP酶阳性的Langerhans细胞进行了数量及形态定量研究。结果 :研究发现各期中Langerhans细胞的数量不同 ,不典型增生中最少 (P <0 .0 5 ) ,早期浸润癌数量明显增多 (P <0 .0 5 )。图像分析发现病变各期Langerhans细胞的面密度、体密度、形态因子均较正常上皮中有明显改变 (P <0 .0 5 ) ,但与表面基本相同 (P >0 .0 5 )。结论 :Langerhans细胞数量和形态改变可能与人类舌癌的发生、发展有关。

Objective:To study the function of ATP-ase positive Langerhans cells in development of human tongue squamous cell carcinoma.Methods:ATP-ase positive Langerhans cells in normal membrance epiderm,atypical hyperplasia,carcinoma in site,invasive carcinoma was investigated by electronic computer image analyzer.Results:The number of Langerhans cells in atypical hyperplasia was decreased (P<0.05),but the number of langerhans cell in carcinoma in site was increased (P<0.05).The alteration of morphologic of Langerhans cells was noted by electronic computer image anatyzer,including decreasing in surface density and volum density (P<0.05),Increasing of dendricity index (P<0.05),but specific surface was not alterrated.Conclusions:These results show that altertion the number and morphology of Langerhans cell are related with the devolepment of human tongue squamous cell carcinoma.