降脂灵片中决明子定性和定量分析方法的研究

The study on qualitative analysis and quantitative analysis method of Semen Cassiae in JiangZhiLing tablet

目的 :通过对决明子的定性和定量分析来控制降脂灵片的质量。方法 :采用薄层层析法对决明子进行定性分析 ;应用高效液相色谱法 ,采用ODS -C18色谱柱 ,以甲醇∶0.1%高氯酸(80∶20)为流动相 ,检测波长为365nm ,测定决明子中大黄素的含量。结果 :在0.05～0.8mg/L浓度范围内 ,线性关系良好 ,回归方程为Y=32.92 +5391.86X ,r=0.9998 ,平均回收率为98.70 %。结论 :本法简便可靠准确 。

Objective:The quality of JiangZhiLing tablet was controlled based on the qualitative analysis and quantitative analysis of Semen Cassiae.Methods: Qualitative analysis of Semen Cassiae was performed by TLC (thin-layer-chromatograph); HPLC(high-performance liquid chromatograph) was applied to determine the quantity of Emodin in Semen Cassiae at 365nm wavelength, in which ODS-C18 volume, 0.1% methanol flow phase and PCA (perchloric acid) were used. Results: Emodin's detection linear range is 0.05～0.8mg/L. The regression equation is Y=33.92+5391.86X, r=0.9998, average recovery is 98.70%. Conclusion: This method is simple and reliable enough to be used as the method of quality-control.