丙型肝炎医院感染患者及供血员血清病毒含量随访检测

Quantitative Detection of HCV RNA in Sera from Patients with Nosocomial Infection and Donors

目的 研究医院内输血后丙型肝炎病毒 (HCV)感染的临床流行病特点 ,探讨改进供血筛检的方法。方法 以 RT- PCR法对 HCV感染的受血及相关供血血清进行 HCV RNA定量分析 ,并检测 AL T及抗 - HCV。结果 致输血后 HCV感染的供血中 ,HCV RNA平均含量为 10 8.6 5拷贝 / L;抗 - HCV及 AL T的异常检出率及水平随病毒滴度升高而增加。结论 低含量 HCV供血即可导致输血后感染 ,病毒含量与抗 - HCV阳性及 AL T水平有显著正相关 ,采用 AL T加抗 - HCV联合筛检供血可明显降低输血后

OBJECTIVE To determine the clinical epidemiologic features of posttransfusion HCV nosocomial infection and to improve the methods of donor blood screening.METHODS The qualitative and quantitative HCV RNA in sera were tested, ALT and anti HCV in serum were tested simultanaeously.RESULTS In serum of donor blood which caused posttranfusion HCV infection,the mean quantity of HCV RNA was 10 8.65 copy/L.The positive rate of anti HCV in serum was increased with the quantity of HCV RNA.CONCLUSIONS There was a strong correlation between the quantity of HCV RNA and ALT value.Posttransfusion HCV infection could be caused by transfusing blood with low quantity of HCV RNA and exclusion of blood with elevated ALT or positive for anti HCV could reduce the cases of posttransfusion HCV infection.