硬肝复康防治肝硬化的初步实验研究

Experimental Studies on the Preventive and Therapeutic Effects of Yingganfukang on Hepatocirrhosis

目的 :研究复方中药硬肝复康对小鼠实验性肝纤维化的防治作用。方法 :单次或多次给予实验小鼠CCl4 油剂 ,分别造成急性肝损伤和肝纤维化 ;给小鼠静脉注射BCG和LPS造成免疫性肝损伤。检测各组实验小鼠血清ALT、AST、ALP ,并取肝纤维化小鼠肝脏做病理学检查。结果 :CCl4 急性肝损伤实验中 ,硬肝复康 (2g/(kg·d) )组小鼠ALT、ALP( (6153±3491)IU/L、(202±24)IU/L)低于对照组 ( (9275±2744)IU/L、(421±67)IU/L) (P<0 05) ;免疫性肝损伤实验中 ,硬肝复康组ALT、AST( (36 3±13 0)IU/L、(164 4±17 2)IU/L)显著低于对照组 ( (83 2±52 6)IU/L、(235 5±73 4)IU/L) (P<0 01) ;实验性肝纤维化实验中 ,硬肝复康组ALT、ALP( (1100±342)IU/L、(166±54)IU/L)显著低于对照组 ( (1638±336)IU/L、(328±128)IU/L) (P<0 01)。病理检查显示 ,硬肝复康组肝脏纤维化程度明显较轻。结论 :硬肝复康对慢性肝损伤和肝纤维化具有较好防治作用。

OBJECTIVE:The preventive and therapeutic effects of a compound traditional Chinese medicine preparation,Yingganfukang(YGFK),on mice experimental liver fibrosis were studied METHODS:The acute liver damage and fibrosis rat models were established by giving single or multi-dose of CCl4 orally and the liver dysimmunity model was established by giving BCG and LPS intravenously Rats were divided into YGFK group and control group The serum ALT,AST and ALP of the mice were examined The liver tissues taken from the liver fibrosis mice were committed to pathological examination RES_ULTS:The serum ALT,ALP of the YGFK(2g/kg·d) group and control group were (6 153±3 491)IU/L、(202±24)IU/L and (9 275±2 744)IU/L、(421±67)IU/L,respectively in the acute liver damage mice with significant differences(P<0 05) The ALT and AST of the YGFK group and control group were (36 3±13 0)IU/L、(164 4±17 2)IU/L and (83 2±52 6)IU/L、(235 5±73 4)IU/L,respectively in the immune liver damage mice with significant differences(P<0 01) The ALT and ALP of the YGFK group and control group were (1 100±342)IU/L、(166±54)IU/L and (1 638±336)IU/L、(328±128)IU/L,respectively in the liver fibrosis mice(P<0 01) The severity of liver fibrosis was slight in the YGFK group CONCLUSION:The preventive and therapeutic effects of Yingganfukang on chronic liver damage and liver fibrosis are reliable