HPLC-紫外法测定人血浆中芬太尼浓度

Determination of Concentration of Fentanyl in Human Plasma by HPLC With UV Detection

目的 :建立高效液相色谱法 -紫外检测器测定人血浆中芬太尼浓度的方法。方法 :本实验采用外标法 ,以Shim -PackCLC -ODS(6 0mm×150mm ,5μm )为固定相 ,含0 015mol/LNaH2PO4 的乙腈 -水溶液 (30∶70 ,v/v)为流动相 ,流速1 5ml/min ,紫外检测波长195nm。结果 :标准曲线在2 0～100ng/ml范围内线性关系良好 (r=0 999) ,最低检测浓度为1ng/ml,方法回收率为(91 70±4 70) % ,提取回收率为 (97 38±3 69) % ,日内变异RSD (6 50±2 79) % ,日间变异RSD (6 70±3 04) %。结论 :本方法简便 ,准确 ,检测浓度低 ,能够满足血浆中低浓度芬太尼的测定及临床药代动力学研究的要求。

OBJECTIVE:To determine the concentration of fentanyl in human plasma by HPLC with ultraviolet detection METHODS:External standard method was used The eluation of fentanyl was carried out by shim-Pack CLC-ODS(6 0mm×150mm,5μm)column with 0 015mol/L NaH2PO4 acetonitril-H2O(30∶70,v/v)as the mobile phase,and detection wavelength was 195nm RESULTS:Calibration curve for fentanyl was linear in the range of 2 0～100ng/ml(r=0 999) The detection limit was 1 0ng/ml The within-day RSD was (6 50±2 79)%,the between-day RSD Was (6 70±3 04)% The method recovery was (91 70±4 70)% The extraction recovery was (97 38±3 69)% CONCLUSION:This method was quick and accurate for the determination of fentanyl in human plasma and useful for pharmakinetic study of fentanyl