摘要
目的 :建立薄层扫描法测定降糖丸中葛根素含量的方法。方法 :固定相为硅胶GF254,流动相为氯仿 -甲醇 -水 (7∶2 5∶0 25) ,检测波长为254nm ,参比波长为370nm。结果 :葛根素在0 4μg～2 0μg 范围内呈良好的线性关系 ,平均回收率为99 4 %(n=5) ,RSD为1 37 %。结论 :本方法准确、可靠 ,可用于测定降糖丸中葛根素的含量。
OBJECTIVE:To develop a method of thin-layer chromatographic scanning to determine the content of puerarin in Jiangtang pill METHODS:Silica GF254 thin-layer was used,and the mobile phase consisted of chloroform-methanol-water(7∶2 5∶0 25) with the detection wave-length of 254nm and the control wave-length of 370nm RESULTS:The linear range of puerarin was 0 4～2 0μg The mean recovery was 99 40%(n=5) with RSD of 1 37% CONCLUSION:This method is accurate,reliable and can be used for the determination of puerarin in Jiangtang pill
出处
《中国药房》
CAS
CSCD
2001年第5期299-300,共2页
China Pharmacy
关键词
薄层扫描法
葛根素
降糖丸
中药
thin-layer chromatographic scanning
puerarin
Jiangtang pill
