黄连解毒汤含药血清对血管内皮细胞增殖及粘附中性粒细胞能力的影响

Influence of Huang Lian Jie Du Decoction-Containing Serum on Neutrophil-Endothelial Cell Adhesion

以体外培养的人脐静脉内皮细胞 (HUVEC)为模型 ,应用MTT和蛋白染料染色法 ,观察给药一次及连续给药 3d后0 5、1、1 5、2、2 5h所取黄连解毒汤 (HLJDT)含药血清对HUVEC增殖及粘附中性粒细胞 (PMN)能力的影响 ,以探讨HLJDT含药血清制备的最佳取血时间点及其抗炎作用机制。结果 :①与对照血清相比 ,40 %及 2 0 %浓度的含药血清对HUVEC均有不同程度的抑制增殖作用 ,而 10 %浓度时无明显抑制作用 ;②各点的HLJDT含药血清均有一定的抑制HUVEC PMN粘附的作用 ,且与取血时间点存在一定的时效关系。其抑制细胞粘附的最佳时间点为连续给药 3d组的 1 5h左右。结论 :HLJDT含药血清能抑制HUVEC PMN粘附 ,可能是HLJDT抗炎作用的机制之一。

In order to observe Huang Lian Jie Du Decoction (HLJDD)'s anti-inflammation mechanism and the best time to collect its herbage-containing serum from rats,human umbilical vein endothelial cells (HUVEC) cultured in vitro and protein staining was used to study the effect of serum on neutrophil-endothelial cell adhesion by treated with HUVEC for 4h、12h、24h,the serum was collected on 0.5h 1.0h 1.5h 2.0h 2.5h after treating with HLJDD (one dose once a day while another dose twice a day for three days).The results showed that the herbage-containing serum could decrease the cell adhesion in some degree with time-effect relationship.Compared to the control group,the best time to collect its serum was 1.5h around by dosed twice a day for three days.The HLJDD herbage-containing serum could decrease the neutrophil-endothelial cell adhesion,and inhibit neutrophil transuding from vessel,which might be one of HLJDD's anti-inflammation mechanisms.