苏云金杆菌发酵条件的研究——Ⅱ.83002菌株在14L发酵罐发酵条件与制剂生产

STUDIES ON THE FERMENTATION OF BACILLUS THURINGIENSIS II .FERMENTATIVE CONDITION OF 14L FERMENTOR AND PREPARATION PRODUCTION

以苏云金杆菌83002菌株在14L发酵罐条件下研究表明:培养温度和搅拌速度对菌数表现出一定的相关性,但对培养基pH变化规律影响不大;培养基灭菌前的初始pH值高低对发酵过程中pH变化规律有一定影响,培养温度、搅拌速度、培养基灭菌前pH值高低对发酵过程中培养基C、N变化的规律均表现出一定影响。上述结果,为苏云金杆菌发酵条件参数提供了依据,确立了各种剂型的制备工艺。

In this paper, the relationship between the culture temperature, speed of agitation, the pH befere autoclaving with the varies of N, C, pH of medium and bacterial numbers during fermentation process were studies by 14L fermentor. The culture temperature and speed of agitation are in relation to bacterial numbers. The starting pH befere autoclaving of medium bear a relation to pH variation during fermentative process. The culture temperature, speed of agitation and starting pH value have an effct on N, C of the medium. Technological process of preparation type of Bacillus thuringiensis has been defined.