21种抗体对胚胎不同器官蛋白质表达的研究

STUDY ON PROTEIN EXPRESSION OF DIFFERENT EMBRYONIC ORGANS WITH 21 KINDS OF ANTIBODIES BY IMMUNOHISTOCHEMISTRY

目的研究蛋白质表达规律及其与胚胎发生的联系。方法取引产 19周龄胎儿的 7种脏器 ,即肝、脾、肾、胃、皮肤、肺和胸腺做石蜡包埋 ,常规切片 ,胚胎 7种器官对着 2 1种抗体做免疫组织化学染色 ,苏木精复染 ,依据显微照相和镜下观察确定细胞类型、抗体染色强度及部位 ;观察分析胚胎不同器官细胞中蛋白质的表达情况及其和分化的联系。结果蛋白质组合实际表达频率高于其随机表达理论值 ,有非常显著性意义 (P <0 .0 1) ;起源相同但分化不同的细胞 ,往往带有相同的一组蛋白质。结论多组蛋白质有成组表达倾向 ,蛋白质的成组表达与细胞发生原基有关 ,但是细胞发生的同源性不是蛋白质成组表达的惟一原因。

ObjectiveTo investigate rule of protein expression and relation between protein expression and embryogenesis.MethodsSeven kinds of embryonic organs including liver, spleen, kidney, stomach, skin, lung and thymus were taken from a 19 week embryo by induced delivery. These tissues of different organs were embeded with paraffin, routine sections were performed. Then 21 kinds of antibodies were used to assay the components among the seven types of organs by immunohistochemistry. After this process, hematoxylin staining followed.Cell type, location and intensity of staining were observed and analyzed through microscope and its photos to find rule of protein distribution from the different embryonic organs and its relation with embryogenesis.ResultsThere existed a significant differencs in statistics between the actual frequency and theoretical frequency of protein expression(P< 0.01 ).The differential cells from the same ancestor generally held the same groups of proteins.ConclusionMany proteins tend to distribute in groups in cells.This phenomenon has some relation with cell genesis, however, the cell isogenesis is not the only reason for protein distribution grouped.