异种淋巴细胞混合培养时T淋巴细胞分泌细胞因子的格局

Cytokine profiles of T lymphocytes in xenotransplantation

目的 研究异种淋巴细胞混合培养时T淋巴细胞亚群分泌细胞因子的格局。方法 用单向异种混合淋巴细胞反应体系研究人 猪间细胞反应的细胞因子分泌格局。结果 γ干扰素的mRNA与蛋白质均强表达 ,而白细胞介素 4(IL 4)均无表达。初次混合淋巴细胞反应时 ,IL 10的mRNA弱表达 ,但是猪抗原特异性T淋巴细胞系中未检测到IL 10mRNA表达。结论 异种淋巴细胞混合培养时以Ⅰ型细胞因子分泌为主 。

Objective To study the cytokine profiles of T lymphocytes in xenotransplantation. Methods An one way heterogenous mixed lymphocytic reaction (MLR) system was used to study the secretory profiles of the cytokines in mini pig to human. Results mRNA and protein of IFN γ were strong expressed, but IL 4 expression was not detectable. In primary MLR, IL 10 mRNA was weakly expressed, but in the antigen specific T cell lines of pig, no IL 10 protein expression was detectable in MLR. Conclusions The type I cytokine profiles are mainly involved in the xeno cell reaction. The hetreogeneitic cytokine profiles are same as that of allotransplantation.