摘要
目的 研究小鼠胰岛分离和移植的方法。方法 在Gotoh等所介绍的小鼠胰岛分离方法的基础上作了一些改进 ,由原来经胆总管注入胶原酶消化液改为由胆囊注入 ,并在消化液和Ficoll分离液中加入了胰酶抑制剂和BSA。结果 使分离纯化后的胰岛产量由原来方法的 41.7± 13.2个提高到了 2 6 6 .5± 32 .1个 (P <0 .0 1) ,活性在 95 %以上 ,除了少量导管外几乎不含腺泡组织。结论 改进后的方法可以在肉眼条件下注入消化液 ,而不需要解剖显微镜 ,既方便了操作又提高了成功率 ;避免了整个消化和分离过程中胰酶对胰岛的消化作用 ,提高了得率 。
Objectives To study of the method for isolation and transplantation of mouse islets. Methods The method for isolation of mouse islets described by Gotoh G was modified. The solution of digestion was injected not through the common bile duct but through gallbladder. Soybean trypsin inhibitor and BSA were added into the digestive and Ficoll separation solutions. Results The yield of islets was increased from 41.7 ± 13.2 to 266.5 ± 32.1 ( P < 0.01 ). Islet viability was more than 95?%. Among the purified islets, there was no exocrine tissue but few ductal fragments. Conclusions By the improved method, digestive solution could be injected into pancreas without inverted microscope, which made manipulation easier and more successful. Having avoided the digestive effect of trypsin on islets, the yield of islets was increased and good repetitiousness was obtained.
出处
《中华器官移植杂志》
CAS
CSCD
北大核心
2001年第3期176-178,共3页
Chinese Journal of Organ Transplantation
基金
国家自然科学基金资助项目!( 39770 70 5)
关键词
小鼠
胰岛移植
细胞分离
实验研究
Mice
Islets of langerhans
Transplantation
Cell separation