大鼠表皮角化细胞Cx43表达和细胞增殖活性的关系

The relationship between the expression of Cx43 and the proliferation of keratinocytes in epidermis of rats

目的 :研究大鼠前肢表皮角化细胞Cx43的表达和细胞增殖活性的拓扑学分布特点及二者间的关系。方法 :常规石蜡切片 ,利用Cx43和增殖细胞核抗原 (proliferatingcellnuclearantigen ,PCNA)免疫组化法 ,分析单位面积表皮细胞中Cx43表达的长度 (LA)和PCNA阳性细胞核的百分率。结果 :在水平方向上 5mm范围内 ,表皮细胞Cx43的表达随部位而异 ,且在距离始端大约 3.0～ 4.5mm处Cx43表达丰富。同时 ,在水平方向上 10mm范围内 ,角化细胞的增殖活性随部位而异 ,且增殖活性类似的角化细胞各自聚集成群 ,其直径大小约为 10 -3 m数量级 ;并在距离始端约 3.0～ 4.0mm处是角化细胞增殖活性低的位置。结论

SUMMARY Objective: To study topographic characters of the expression of Cx43 and the proliferation activity of keratinocytes on the upper legs of rats and to explore the relationship beween them. Methods: By rutine paraffin section , immunohistochemical techniques of Cx43 and PCNA were used to study the length of the expression of Cx43 in a unit area of epidermis(L A) and the percentage of proliferating cell nuclear antigen(PCNA)positive nucleus(%). Results: Within the range of 5 mm in the horizonal direction, the expression of Cx43 in epidermis varied according to the locations of keratinocytes and Cx43 was highly expressed which was about 3.0～4.5 mm from the start point. At the same time, within the range of 10 mm in the horizonal direction, the proliferation activity of keratinocytes was different from site to site and the kerationcytes with similar proliferation activity were clustered as groups, the scale of which was about 10 -3 m; One of the sites, with low proliferation activity, was about 3.0～4.0 mm from the start point. Conclusion: This study suggested that the keratinocytes with low proliferation activity had high expression of Cx43.