周期性牵张力对人牙周膜细胞中成骨样细胞表型碱性磷酸酶和骨钙素mRNA表达的影响

Effect of cyclical mechanical force on expression of mRNAs for osteoblast like marker proteins in human periodontal ligament cells in vitro

目的 :了解机械力介导的牙周膜细胞中成骨样细胞特性的改变 ,以利于深入研究正畸牙齿移动的机制。方法 :应用地高辛标记寡核苷酸探针进行细胞原位杂交 ,检测在间歇性机械牵张力作用下人牙周膜细胞中的成骨样细胞表型蛋白碱性磷酸酶和骨钙素在mRNA转录水平的表达改变。结果 :间歇性牵张力作用增强人牙周膜细胞碱性磷酸酶和骨钙素基因的表达。结论

SUMMARY Objective: To study the role of mechanical stress on osteoblast like characteristics expressed by human periodontal ligament cells in vitro. Methods: Human periodontal ligament cells (hPDLC) were cultured by explant method. We investigated the gene expression for osteoblast like marker proteins in periodontal ligament stimulated by cyclical mechanical force in vitro by in situ hybridization with non radioisotopic oligonucleotide probe for alkline phosphatase (ALP) and osteocalcin (OCN). Results: Human periodontal ligament cells exhibited a stronger signal for ALP mRNA stimulated by cyclical mechanical force, and exhibited a moderately intense positive signal for OCN mRNA —OCN was a marker of differentiated osteoblast like cells. Conclusion: These findings suggest that mechanical force plays role in differentiation from hPDLC to osteoblast like cells.