幽门螺杆菌诱导人胃上皮癌细胞株BGC-823环加氧酶-2的合成

Helicobacter pylori induces the synthesis of cyclooxygenase 2 in human gastric epithelial carcinoma cell line BGC 823

目的 :研究幽门螺杆菌对人胃上皮细胞环加氧酶 1和环加氧酶 2合成的影响。方法 :实验选用VacA(+)和CagA(+)的国际标准幽门螺杆菌菌株NCTC116 37和人胃上皮癌细胞株BGC 82 3。环加氧酶的合成用免疫蛋白印迹法 (WesternBlotting)测定。结果 :幽门螺杆菌菌体超声提取物对BGC 82 3细胞环加氧酶 2的合成有明显的诱导作用。BGC 82 3细胞环加氧酶 2的含量在幽门螺杆菌提取物作用 1h后明显增加 ,并持续至少 6h。幽门螺杆菌提取物对BGC 82 3细胞环加氧酶 1的合成没有影响。幽门螺杆菌脂多糖成分对BGC 82 3细胞环加氧酶 2的合成没有影响。结论 :幽门螺杆菌可直接诱导BGC 82 3细胞环加氧酶 2的合成 。

SUMMARY Objective: To investigate the effect of Helicobacter pylori (H. pylori) on the synthesis of cyclooxygenase 1 (COX 1) and cyclooxygenase 2 (COX 2) in gastric epithelial cells. Methods: a VacA(+) and CagA(+) international standard H. pylori line NCTC11637 and a human gastric epithelial carcinoma cell line BGC 823 were used. Western Blotting was applied to detect the synthesis of cyclooxygenase. Results: The content of COX 2 protein increased obviously after the cells were incubated with H. pylori sonicating extract for 1 h and the increase lasted for at least 6 h whereas the content of COX 1 protein did not change during the incubation with H. pylori extract. H. pylori lipopolysaccharide (LPS) had no effect on COX 2 synthesis. Conclusion: H. pylori stimulated the synthesis of COX 2 in BGC 823 cells and the effect was LPS independent.