氧化型胆固醇诱导兔血管平滑肌细胞凋亡

Oxysterols induced apoptosis in rabbit vascular smooth muscle cells

目的 :研究氧化型胆固醇 (Triol与 2 5 OH)对兔主动脉血管平滑肌细胞 (vascularsmoothmusclecell,VSMC)凋亡的诱导 ,并观察其时效与量效关系。方法 :原代培养兔VSMC ,利用光镜、电镜、DNA电泳及TUNEL法作为凋亡检测法。结果 :光镜、电镜下可见典型的细胞凋亡形态学改变 ,电泳示“DNAladder” ,TUNEL法示正常兔VSMC凋亡率为 3.6 2 % ,用Triol和 2 5 OH不同质量浓度 (5、10、15、2 0mg·L-1)分别处理细胞 2 4h后 ,凋亡率明显升高 ;用Triol和 2 5 OH(15mg·L-1)分别处理细胞 0、12、2 4、36h后 ,凋亡率随时间延长而增加。结论 :氧化型胆固醇 (Triol与 2 5 OH)可以诱导血管平滑肌细胞发生凋亡 。

SUMMARY Objective:To investigate the apoptosis of rabbit vascular smooth muscle cells (VSMC) induced by oxysterols and observe the time and dose effect. Methods: Light miroscope, electron microscope, DNA agarose gel electrophoresis and TUNEL were uesed to detect the apoptosis of rabbit aortic VSMC. Results:The characteristic morphological features of apoptosis were observed under light and electron microscope; DNA electrophoresis showed “DNA Ladder”; TUNEL showed the apoptotic rate of normal rabbit VSMC was 3.62%. While treated with either Triol or 25 OH by different dose (5, 10, 15, 20 mg·L -1 ) and at different times (0, 12, 24, 36 h), the apoptotic rate increased significantly. Conclusion: Both Triol and 25 OH can induce apoptosis of vascular smooth muscle cells in a dose and time dependent manner.