PCD-hIL-2合并加味当归补血汤促进骨髓移植后免疫重建

PCD- hIL -2 and Chinese medicine accelerate the immunofunction reconstitution in bone marrow transplantation mice

目的 :采用近交系小鼠 ,建立自体骨髓移植后免疫缺损模型 ,并尝试应用PCD hIL 2注射和中药促进骨髓移植后免疫功能重建。方法 :用BALB/C小鼠亚致死剂量照射后行同系骨髓移植 ,并用裸基因PCD hIL 2注射与中药加味当归补血汤治疗免疫缺损。实验分组 :A ,中药 +PCD hIL 2 ;B ,PCD hIL 2 ;C ,中药 +hIL 2 ;D ,中药 ;E ,hIL 2 ;F ,单纯BMT ;G ,正常对照 ;H ,放射对照。结果 :骨髓移植后 10d ,发现A、B、C、D组与E、F组相比 ,下述参数差异有显著性 (P <0 .0 1) :(1)脾脏、胸腺细胞总数明显增加 ;(2 )体内NK细胞杀伤活性明显升高 ;(3)脾细胞、胸腺细胞、骨髓细胞对丝裂原的反应性增高 ;(4 )混合淋巴细胞反应加强 ;(5 )体内脾细胞产生IL 2水平及对外源性IL 2的反应性升高 ;(6 )骨髓CFU GM数增加。结论 :PCD hIL

SUMMARY Objective: In order to study how to accelerate the reconstitution immunofunction in BMT mice, first of all, we established a immunodeficiency model of BMT in BALB/C mice. Then BMT mice were injected with PCD hIL 2 directly into skeletal muscle, and treated with traditional Chinese medicine. Methods: The experiment groups are designed as(A)Chinese medicine + PCD hIL 2;(B)PCD hIL 2;(C)Chinese medicine +hIL 2;(D)Chinese medicine;(E)hIL 2;(F)BMT;(G)normal control;(H)radiation control. Results: We compared groups A B C D to E or F groups, found(1)The splenocytes/thymocytes count increase obviously.(2)Killing activity of NK cell rises obviously in vivo .(3)The response of splenocytes、thymocytes、BM cells to mitogen goes up.(4)The reactivity of splenocytes to foreign IL 2 goes up. (5)CFU GM count is increased. Conclusion: The expression of hIL 2 is very low by nude DNA injection ,but it is enough to have biological function and therapeutic effect .If only Chinese medicine was applied, the immunological condition was obviously recovered.