大鼠早期放射性脑损伤中星形胶质细胞的病理学改变

The pathological changes of astrocytes in the rat brain radiation injury model

目的 通过对脑组织中星形胶质细胞病理学改变的观察 ,研究放射性脑损伤的发病机理。方法 利用已建立的大鼠早期放射性脑损伤的实验模型 ,用胶质纤维酸性蛋白免疫组织化学染色标记星形胶质细胞的方法 ,观察大脑海马区中星形胶质细胞形态与数量的变化 ,并分析照射剂量、照射后时间间隔的影响。结果 受照射侧海马中的阳性细胞表现出胞体肥大、突起增多与延长等改变 ,在剂量增加和观察时间延长的条件下 ,2 0Gy照射后 3个月和 30Gy照射后 1个月与 3个月的三个组别中阳性细胞数有显著增加。结论 随着照射剂量的加大和观测时间的延长 。

Objective To investigate the pathogenesis of brain radiation injury by observing the pathological changes of astrocytes in the hippocampus region of rat brain radiation injury model.Methods The brain sections were immunohistochemical stained for glial fibrillary acidic protein (GFAP),which was used to label astrocytes.The morphological characters and the number of GFAP cells in hippocampus were recongnized,and the data were analyzed by the Student’s test.Results The astrocytes proliferation was revealed.The relative number of GFAP positive cells increased in the hippocampus.The increase was dose and time course related.Conclusion The pathological changes of astrocytes suggest a possible association in the pathogenesis of brain radiation injury,just as the other tissue components.