透明质酸联合碱性成纤维细胞生长因子对韧带细胞增殖的影响

THE INFLUENCE OF HYALURONIC ACID AND BASIC FIBROBLAST GROWTH FACTOR ON THE PROLIFERATION OF LIGAMENTOUS CELLS

目的 观察透明质酸 (HA )和碱性成纤维细胞生长因子 (b FGF)对内侧副韧带和前十字韧带细胞增殖行为的影响。方法 培养成年新西兰白兔内侧副韧带和前十字韧带细胞 ,在培养液中分别加入 HA、b FGF或两者的复合物 ,以 MTT方法测定细胞的增殖行为。结果 单独使用 HA对前十字韧带细胞的增殖作用不明显 ,对内侧副韧带细胞的增殖有一定的促进作用。 b FGF在 1ng/ml时即对两种细胞均具有显著的促增殖作用 ,其浓度达 5 0 ng/ml时 ,促进作用最大。10 0μg/ml HA与 10 0 ng/m l b FGF联合使用时 ,对两种细胞增殖的促进作用较单独使用 HA或 b FGF强。结论 HA可以作为 b FGF的载体联合使用以促进韧带创伤愈合。

Objective To observe the effects of hyaluronic acid (HA) and basic fibroblast growth factor(bFGF) on the proliferation of the cells from medial collateral ligament(MCL) and anterior cruciate ligament(ACL) cells. Methods The MCL cells and ACL cells of mature New Zealand white rabbit were cultured, while HA, bFGF or HA and bFGF were added to the cell culture media, the cellular proliferation was assayed by MTT method. Results HA only had no effect on the preoliferation of ACL cells, but had a small stimulatory effect on the proliferation of MCL cells. The addition of 1 ng/ml bFGF enhanced the proliferation of both MCL and ACL cells significantly, and this enhancement was maximal in the concentration of 50 ng/ml. However, the enhancement of proliferation of MCL and ACL cells could be achieved when the combination of HA in concentration of 100 μg/ml and bFGF in concentration of 100 ng/ml. Conclusion It is evident that bFGF can enhance the proliferation of the ligament cells. HA can maintain the normal growth of ACL cells with no effect on the proliferation of the cells, while HA has a small stimulatory effect on the proliferation of MCL cells. However, when bFGF is coordinated with HA, more improvement of cellular proliferation can be achieved. HA can be used as a potential carrier for bFGF to enhance the healing of ligamentous tissue injuries.