中药白龙与环六亚甲基双已酰胺对人胃癌不同周期细胞生长与表型调节的共性研究

Regulative Effects of Bailong and Hexamethylene bisacetamide on Growth and Phenotype of Human Gastric Cancer Cells in Cell Cycle

目的 :对比研究中药复方白龙与西药诱导分化剂环六亚甲基双已酰胺 (Hexamethylenbisace lamide,HMBA)对人胃癌MGC80 3不同周期细胞的生长与表型调节的共性作用。方法 :采用高压笑气加胸腺嘧啶脱氧 (TdR)双阻断法将MGC80 3细胞分别同步于G1、S、G2 、M 4个时相 ,用中药复方白龙和HMBA分别处理各时相细胞。结果 :实验结果表明中药白龙或HMBA对MGC80 3不同周期细胞具有显著的增殖抑制作用 ,抑制率达 4 6 2 %～ 75 3%。细胞在软琼脂上的生长势态和细胞周期中的增殖抑制相似 ,但在S与G2 期中中药白龙的作用优于HMBA。与此同时细胞质微丝组装 ,除M期外 ,均显著增加。结论 :中药白龙与HMBA对人胃癌MGC80 3各时相细胞的生长与分化表型作用具有明显的共性特征 ,为促进中西医结合提供理论认识基础。

Objective: To investigate and compare the common effect of Bailong, a Chinese herbal preparation, and Hexamethylene bisacetamide (HMBA), a western cell differentiation inducer, on growth regulation and phenotype of human gastric cancer (MGC80 3) cells in different phases of cell cycle. Methods: Synchronized MGC80 3 cells in monolayer cultures with nitrous oxide under high pressure were double blocked with overdosage of TdR in the 4 cellular phases (G 1, S, G 2 and M), and then collected and treated with Bailong and 5 mmol/L HMBA respectively. Results: Both medicines could suppress the cell proliferation, soft agar cloning growth and microfilament assembling of different phases of cells, the effect on cells of G 1 phase was the most significant one. Conclusion: Bailong and HMBA had significant common characteristics in inducing cell differentiation of MGC80 3 cells in different phases of cell cycle.