家鱼精液超低温冷冻保存研究 Ⅰ.防冻稀释液的筛选

STUDY ON CRYOPRESERVATION OF FISH SPERMATOZOA

本文对草鱼(Cenophoryngodon idellus)、鲢鱼(Hypophthalmichths molitrix)和青鱼(Mylopharyngodon piceus)精液冷冻保存的防冻稀释液进行了筛选。实验结果表明,由0.3%NaCl、6.6%～6.8%葡萄糖(G)、0.05% NaHCO_3和10%DMSO组成的防冻稀释液的冷冻效果好。冻精在液氮(-196℃)中保存1～3年后,复苏率达60%～75%。冻精受精率分别为:草鱼73.8%～77.6%、鲢鱼72.4%～79.0%、青鱼70.3%～78.5%;孵化率依次为82.8%～91.5%,83.6%～91.7%和63.8%～80.8%。这一试验结果接近或达到对照组受精率和孵化率的水平。

This paper reports the results of sperm cryopreservation of fishes:Cenoph-oyngodon idellus,Hypophthalmichthys molitrix,Mylopharyngodon piceus.Exten-der is composed of NaCl(0.3%),Glucose(6.0%～6.8%),NaHCO_3(0.05%)andDimethyl sulfoxide(DMSO,10%),Survival rate of fish sperm above stored inliquid nitrogen(LN_2,-196℃)is 60%～75%.The fertilization rates of cryoprese-rved sperm after stored in LN_2 one to three years are 73.8%～77.6%(C.idellus),72.4%～79.0%(H.molitrix)and 70.3%～78.5%(M.piceus).Fry hatching ratesare 82.8%～91.5%,83.6%～91.7% and 63.8%～80.8% respecrively.