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快速去除黑斑蛙和牛蛙未受精卵胶膜和卵黄膜的一种简便方法

A SIMPLE METHOD OF DISSOLVING THE EGG-JELLY AND VITELLINE MEMBRANE OF UNFERTILIZED EGGS FROM RANA NIGROMAGULATA AND RANA CATESBEIANA
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摘要 本文介绍了一种快速除去牛蛙和黑斑蛙未受精卵胶膜和卵黄膜的方法,人工催情,从泄殖腔内挤取未受精卵,用0.2M巯基乙醇和0.2%~0.5%胰蛋白酶处理.在20℃左右室温下,15~20min能使胶膜和卵黄膜全部溶解,不需人工辅助剥离便可获得大量裸卵. The present paper discribed a simple and convenient method of dissolvingegg-jelly and vitelline membrane of unfertilized eggs from Rana catesbeiana andRana nigromaculata.Just after unfertilized eggs were obtained from the urogenitelcavity of the frogs,Rana catesbeiana and Rana nigromaculata,they were imm-ersed into 0.2 M mercaptoethanol,then washed with phosphate buffer and Hol-freters' solution and transferred into 0.2%~0.5% trypsin dissolved in Holfreters'solution.At about 20℃,the total time of dissolving all of the egg-jelly and vi-telline membrane were 15 to 20 minutes.
出处 《湖南师范大学自然科学学报》 CAS 1991年第4期351-353,共3页 Journal of Natural Science of Hunan Normal University
基金 国家自然科学基金
关键词 胶膜 卵黄模 育种 牛蛙 frog unfertilized-egg egg-jelly vitelline membrane
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