从葡萄皮渣中提取白藜芦醇的研究

STUDIES ON THE EXTRACTION OF RESVERATROL FROM GRAPE SKIN AS A FUNCTIONAL SUBSTANCE

对功能性物质ＲＰＨＳ（以白藜芦醇为主成分的多羟茎芪类物质）的提取与分离、结构与含量分析进行了初步研究。确定了乙酸乙酯提取ＲＰＨＳ的条件：浸提时间４０ ｍ ｉｎ、浸提温度８０ ℃、料液比１∶４０、浸提两次；硅胶柱层析精制条件：甲醇∶水（４∶１）的溶液的理想洗脱剂。经ＵＶ、ＩＲ 初步鉴定，证明提取物为ＲＰＨＳ，经ＴＬＣ及ＨＰＬＣ分析，确定ＲＰＨＳ中的６ 种成分：ｔｒａｎｓ－ｒｅｓｖｅｒａｔｒｏｌ、ｃｉｓ－ｒｅｓｖｅｒａｔｒｏｌ、ｔｒａｎｓ－ｐｉｃｅｉｄ 和ｃｉｒ－ｐｉｃｅｉｄ，另两种组分未见报导。

In this paper the extraction & separation,structure identification & content determination of RPHS has been carried out.The orthogonal test indicatd the optimum extraction condition of RPHS was 80 ℃,1∶40(grape pomace:ethylacetate),40 min,and extracted twice.Purified experiments with silica gel column showed 80% methanol was the ideal eluant.From the datum of infrared & UV spectra,it can be demonstrated that the extract was really RPHS.Six ingredients of purified RPHS were detected with HPLC.Four of them have been identified.They were trans resveratrol,cis resveratrol,trans piceid & cis piceid,Others have not been reported.