D-核糖提取工艺的研究

STUDY ON THE SEPARATING PROCESS OF D RIBOSE

核糖发酵液用饱和醋酸铅除去菌体、杂蛋白，然后通过ＡＢ－８ 树脂进行脱色，脱色液分别经过００１×７，２０１×７ 树脂脱盐后，经过浓缩，于２０１×４ 硼酸型阴离子交换树脂进行分离。以０．０２ ｍ ｏｌ／Ｌ四硼酸钠缓冲液为洗脱液，将其中核糖流分浓缩、加甲醇除去硼酸根后，加乙醇减压浓缩得到白色核糖固体。

The culture medium removed microbe and protein by adding about 10% saturated PbAc.Treated with AB 8.001×7 and 201×7 resins to decolor and desalt,then passed through 201×4 anion exchange resins (BO - 3),washed with 0.01 0.02 mol/L Sodium tetraborate to exclude Glucose,pentose solution was concentrated and Boric acid was excluded as methyl borate under reduced pressure,D ribose was crysted with addition of EtoH.