摘要
以CA12 5单克隆抗体固相包被 ,用平衡饱和法与CA12 5、Eu3+标异硫氰酸苄基二乙烯三胺四乙酸 -CA12 5单抗形成三层夹心 ,以 β -二酮体为增强液 ,建立CA12 5的时间分辨荧光免疫分析法。数据采用Logit-Log函数和四参数Logistic函数数据处理程序处理。方法的批内和批间CV分别为 4 .5%和 4 .0 % ,平均回收率 96.7% ,灵敏度 3.3μg L ,可测范围为 16— 2 0 62 μg L。本法与CEA无交叉 ,与AFP有 4 .6%交叉 ,与 β -HCG有 12 .4 %交叉。测定结果与进口IRMA试剂盒相比较 ,相关系数为 0 .999,结果呈高度相关。
A two-site time-resolved fluoroimmunoassay (TRFIA) of CA 125 based on the direct sandwich technique has been developed, with the equilibrium method. The monoclonal antibody (MoAb) against CA 125 was labelled with europium by the help of europium-chelate of diethylenetriaminepentaacetic acid (DTPA). The luminescent enhancement system was an enhancement solution that contained mainly of 2-naphthoyltrifluoroacetone. The intra- and inter- assay CV of the TRFIA were 4.5% and 4.0%, respectively, and the recovery rate was 96.7%, the sensitivity was 3.3μg/mL. The cross-reacting rate with CEA was negligible, and that with AFP and β-HCG was 4.6% and 12.4%, respectively. Compared with the imported IRMA Kit, the correlation coefficient was 0.999.
出处
《核技术》
CAS
CSCD
北大核心
2001年第5期385-388,共4页
Nuclear Techniques
