摘要
通过在发生培养液中添加孕酮以研究外源性孕酮对牛体外受精卵发育的影响。结果表明,适量添加孕酮有助于胚胎的体外发育。其中,10ng/ml孕酮添加组的8细胞胚胎发生率(55％)极显著高于对照组(39.0％)(P<0.01);囊胚发生率(18.9％)亦显著高于对照组(9.6％)(P<0.05)。但这种促进作用是通过混合培养系统中的颗粒膜细胞得以实现的。本试验未发现孕酮对胚胎是否产生直接作用。
The experiment was conducted to study the effect of progesterone(P)supplement in the co-culture medium on in vitro development of bovine follicular oocytes fertilized in vitro.The result showed that proper progesterone addition can improve the development of the bovine embryos in vitro.The proportion of eight-cell embryos and blastocysts in 10ng/ml P group(54.7％;18.9％)were significantly higher than those of the control(39.0％;9.6％)(P<0.01;P<0.05).The improvment was achieved through the granulosa cells in the co-culture systerm.Direct effection of progesterone to embryo development was not found in this test.
