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禽传染性支气管炎——肾变病型病毒的分离和初步鉴定

ISOLATION AND IDENTIFICATION OF THE VIRUS OF AVIAN INFECTIOUS BRONCHITIS-NEPHROSIS FORM
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摘要 从发病雏鸡的肾脏中分离到一株禽传染性支气管炎——肾变病型〔AvianInfectious Bronchitis(IB)——Nephrosis Form〕病毒。经盲传三代接种鸡胚后,出现典型的侏儒胚病变。该病毒能被乙醚、氯仿灭活,经56℃30分钟后被灭活,但能抵抗 pH2 30分钟。在电子显微镜下,病毒粒子直径为103nm,有19nm 宽的囊膜,囊膜上有典型的球状突起呈花冠状。 The virus of avian infectious bronchitis(IB)-nephrosis form had been isolated from the kidney of the chicken associated with the nephrosis.Through 3 serial“blind” passages of inoculation of the 9-to-11-day-old chicken embryo,the stunted embryo of the typical lesion had been found.The virus inactivated by ether and chloroform,and by heating for 30 minutes at 56 c,but it could resist to pH 2 for 30 minutes.Under the electron microscope, the viron was 103 nm in diameter and had characteristic bulbous projections on the envelope of 19 nm in breadth as well as typical U-shaped structure inside the viron.
出处 《上海农学院学报》 1992年第2期127-131,共5页
关键词 传染性支气管炎 肾变病型病毒 侏儒胚 分离 鉴定 Infectious bronchitis(IB) Nephrosis form Stunted embryo
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