急性白血病端粒酶活性与p53蛋白表达规律的研究

RESEARCH OF TELOMERASE ACTIVITY AND p53 PROTEIN IN ACUTE LEUKEMIA

目的 :探讨急性白血病 (AL )患者中端粒酶活性与突变型 p5 3蛋白的表达规律。方法 :采用聚合酶链反应—酶联免疫吸附法 (PCR- EL ISA )对端粒酶活性进行半定量测定 ,用流式细胞仪检测突变型 p5 3蛋白表达。结果 :AL患者端粒酶活性显著高于对照组 (P<0 .0 0 1) ;端粒酶活性在 AL的不同病期有统计学差异 ,其中初治组、复发组均显著高于完全缓解 (CR)组 (P<0 .0 1) ;在治疗敏感组与不敏感组间无统计学差异 (P>0 .0 5 )。 AL患者中突变型 p5 3蛋白阳性率为 19.4% (7/36 ) ,在 AL的不同病期 p5 3阳性率无统计学差别 ;在初治敏感组与不敏感组之间亦无统计学差别 (P均 >0 .0 5 )。端粒酶活性与突变型 p5 3蛋白的表达无关。结论 :端粒酶活性与 AL的病期和发展密切相关 ;突变型 p5 3蛋白在 AL中表达较低 ,对临床预后的判断价值较小 ;端粒酶的激活与 p5 3蛋白表达无相关性。

Objective:Research on the law of telomerase activity and mutant p53 protein expression in acute leukemia (AL).Methods:A semi-quantitative estimation of telomerase activity was detected by the method of PCR-ELISA,and the expression of mutant p53 protein was marked with specific monoclonal antibody and examined by flow cytometry.Rusults:Telomerase activity in AL was significantly higher than that in the control group,P<0.001.In AL,both the relapse group and the denovo group had significantly higher telomerase activity than the complete remission (CR) group,and P>0.01 in both.No significant difference was found between the treatment sensitive group and insensitive group,and P>0.05.The positive rate of mutant p53 protein in AL was 19.4 %(7/36),and no statistical difference was found among the relapse group,denovo group and CR group;and also between the treatment sensitive group and insensitive group.Telomerase activity was unrelated to mutant p53 protein expression.Conclusion:Telomerase activity was closely associated with the different stages and progress of AL.Mutant p53 protein had a low positive rate and limited value on judging clinical prognosis in AL.There was no correlation between telomerase activation and p53 protein expression.