PCR方法测定幽门螺杆菌vacA基因型和cagA基因

PCR Method for Measuring vacA Genotype and cagA Gene of Helicobacter Pylori

目的 :建立幽门螺杆菌vacA基因型和cagA基因的PCR测定方法。方法 :应用多聚酶链反应方法对 6 2例慢性胃炎、消化性溃疡和胃癌患者分离获得幽门螺杆菌菌株的vacA基因型和cagA基因进行测定。结果 :6 2株幽门螺杆菌菌株均具有vacA基因 ,所有菌株的vacA基因型均为sla m2型。cagA基因的总阳性率为 5 6 .45 % ;慢性胃炎、消化性溃疡和胃癌患者分离获得幽门螺杆菌的cagA基因阳性率分别为 5 5 .5 6 %、5 4.17%和 6 3 .6 4% (P >0 .0 5 )。结论 :本研究建立的PCR测定幽门螺杆菌vacA基因型和cagA基因方法敏感性高。

Objective:To set up PCR method for measuring vacA genotypes and cagA gene of H.pylori.Methods:vacA genotypes and cagA gene of 62 H.pylori strains isolated from patients with chronic gastritis,peptic ulcer and gastric cancer were tested by polymerase chain reaction.Results:All 62 H.pylori strains possessed vacA gene and vacA genotypes of all strains were type s1a/m2.Total positive rate of cagA gene was 56.45%;the positive rates of cagA gene of H.pylori strains isolated from the patients with chronic gastritis,peptic ulcer and gastric cancer were 55.56%,54.17% and 63.64%,respectively( P >0.05).Conclusion:There was no correlation among cagA gene and vacA genotypes of H.pylori and various gastroduodenal diseases.