人纤维蛋白胶对大鼠肝损伤的止血护创作用

The hemostatic and protective effect of human fibrin sealant on liver trauma in rats

目的探讨局部应用人纤维蛋白胶 (FS)对实验性肝损伤的止血和护创作用。方法采用大鼠肝表面裂伤 (Ⅰ型 )和部分肝切除断面伤 (Ⅱ型 )模型 ,以自然止血法为阴性对照 ,缝合止血法和凝血酶止血法为阳性对照 ,比较各组间创面止血时间和术后d 1、4、7、14及 5 0光镜下创面愈合情况。结果与自然止血组相比 ,FS组对Ⅰ型肝损伤模型止血时间平均缩短 86 .0 % (P <0 .0 1) ,对Ⅱ型肝损伤模型缩短 79.0 % (P <0 .0 1) ;与凝血酶止血法相比 ,FS组止血时间分别缩短 45 .0 %和 84.0 % (P <0 .0 1和P <0 .0 5 )。肉眼和镜下观察 ,FS组创面愈合较快 ,局部组织损伤较轻 ,术后 5 0d腹腔粘连基本消失。结论FS对大鼠肝损伤有明显止血作用 ,并能促进创伤的愈合。

PurposeThe aim is to investigate the hemostatic and protective effect of fibrin sealant(FS) on liver trauma in rats.MethodsOn the surface of cracked wound(type Ⅰ) and resected wound(type Ⅱ),spraying of FS or thrombin or direct suturation, was used receptively. Natural hemostasis was used as control.During postoperation the hemostatic time and the state of wound healing on 1st, 4th, 7th,14th, 50th day were observed.ResultsCompared with the natural hemostasis,the hemostatical time of the FS group was shorter 86.0%( P<0.01 )in liver trauma type Ⅰ and 79.0%( P<0.01 )in liver trauma type Ⅱ.Compared with the thrombin group,the hemostatical time of the FS group was shorten 45.0%( P<0.01 )in liver trauma type Ⅰ and 84.0%( P<0.05 ) in liver trauma type Ⅱ.The wound surface of FS group was healed faster than that of other groups.ConclusionFS was an effective hemostatic and healing promoter on liver trauma of rats.