转人TNFα基因对肾细胞癌细胞致瘤性的影响

INFLUENCE ON THE TUMORIGENICITY OF THE HUMAN RENAL CELL CARCINOMA (RCC) CELLS TRANSDUCED WITH HUMAN TNFα GENE

目的 :观察转人TNFα基因对肾细胞癌细胞 (RCC)致瘤性的影响 ,为进一步对RCC的基因治疗打下基础。 方法 :将人TNFα基因构建入逆转录病毒载体 ,经包装、鉴定后 ,感染RCC细胞株 786 0 ,ELISA法测转人TNFα基因 786 0细胞上清中TNFα的活性 ,同时 ,将转人TNFα基因的 786 0细胞接种裸鼠 ,观测转TNFα前后RCC细胞致瘤性的变化。结果 :转人TNFα786 0细胞上清TNFα的浓度平均为 (5 0 0 4± 6 2 4) pg/ml,接种裸鼠后无肿瘤长出。结论 :转人TNFα基因使建株RCC失去了致瘤性。

Purpose: To study the influence on the tumorigenicity of the human renal cell carcinoma (RCC) cells by transfection of human TNFα gene. Methods: hTNFα cDNA was inserted into retroviral vector to construct recombinant vector pL(TNFα)SN. The packaged and confirmed retrovirus were infected to RCC cell line. Then, nude mice were injected by 786 0 cell infected with the hTNFα gene or not , and the tumorigenecity of RCC was observed. Results: The mean expression level of TNFα in the supernatant of TNFα transfected 786 0 cell culture medium was (5 004±624) pg/ml. And the nude mice injected with TNFα gene engineered RCC cells had no tumor growth. Conclusion: The TNFα gene engineered human RCC cells lost tumorigenicity.