用限制性内切酶技术区别B病毒与单纯疱疹病毒1型和2型

A Rapid Mathod for Distinguishing B Virus from Herpes Simplexvirus Type 1 and 2 by Restriction Enzyme Technology

进一步从基因组水平鉴定B病毒 147株的实质。采用Hirt上清法从BV147、HSV_1、HSV_2感染细胞中提取病毒基因组并用BamHI进行酶切 ,电泳。结果所获得的BV147的BamHI图谱与国外报道的基本一致。进一步证实了新分离的病毒是B病毒 ,并为克隆B病毒的基因组、构建其物理图谱奠定了基础。

To further identify the nature of BV147 and genotype.A rapid technique,based on restriction_endonuclease analysis of the viral genome is described.It distinguishes B virus from herpes simplex virus type 1 and 2 unambiguously and lays foundation for constructing the physical mapping of the newly isolated B virus.