摘要
采用金合欢 (Acaciadealbata)大树顶芽、幼苗茎尖、种子小苗上胚轴和幼叶作外植体进行组培和快繁研究。结果表明 :用MS + 6 BA 6mg/L +NAA 0 .1mg/L +NaH2 PO430mg/L可提高愈伤组织的出芽率 ,有利于扩大繁殖系数 ,而用MS + 6 BA 2mg/L +NAA 0 .1mg/L +NaH2 PO4 30mg/L可以增加发枝率。珍珠岩 +泥炭 (1∶1)基质可促进小苗后期生长 ,加速成苗。
Acacia dealbata is a famous kind of economical and oremental trees in Australia.In this test,shoot tips taken from big trees and seedlings,little leaves from young plants,epicotyls from seeds were collected as explants that were studied on tissue culture and rapid propagation.The results showed that all four kinds of explants have been induced callus formation.Buds and shoots can be induced on callus except little leaf’s callus in morphogensis.The medium MS+6 BA 6mg/L+NAA 0.1mg/L +NaH 2PO 4 30mg/L may increase sprouting bud rate from callus.However,the medium MS+6 BA 2mg/L+NAA 0.1mg/L+NaH 2PO 4 30mg/L may increase shoot growing rate.Plantlets from test tubes grew better on the growth medium perlite+peat(1∶1)than on other components in acclimatization stage.
出处
《园艺学报》
CAS
CSCD
北大核心
2001年第2期149-152,共4页
Acta Horticulturae Sinica
关键词
金合欢
组织培养
快速繁殖
成苗
Acacia dealbata
Tissue culture
Rapid propagation
Acclimatization
