摘要
研究了高效液相色谱法测定牛乳中左旋咪唑残留量的方法。牛乳样品通过调节pH值和加甲醇沉淀蛋白质,提取液用Sep-PakC18净化柱净化,SpherisorbC18色谱柱分离,紫外检测器检测,外标法定量。分析了左旋咪唑在Sep-PakC18净化柱上的净化条件和SpherisorbC18色谱柱上的分离条件。本方法检测低限为10μg/kg。对添加10,50,200μg/kg左旋咪唑的牛乳样品,回收率为80.1%~84.2%,变异系数(CV)为7.2%~9.6%。
:A method for determination of residues of levamisole in milk by HPLC is reported.The protein of milk sample is precipitated by adjusting pH and adding methanol.Filtrate is cleanedup by Sep-PakC_18Cartridges.The levamisole is separated on Spherisorb C_18 column,and is detected by UV detector on 230 nm and is quantified by external standard.The detecttion limits of the levamisole is 5μg/kg.The recoveries are 80.1%~84.2%and the variation coefficients are 7.2%~9.6%.
出处
《中国乳品工业》
CAS
北大核心
2001年第1期24-26,共3页
China Dairy Industry