花生品种间硝酸还原酶活性与其耐肥性的研究

A Study on Nitrate Reductase Activity and Nitrogen Response in Groundnut

花生主茎叶定型后的功能期,硝酸还原酶活性(NRA)很高,并可随其功能期保持一段时间后而下降.在整个生育期内NRA在苗期出现一次明显的高峰.苗期NRA与品种耐肥性呈负相关.经对大田栽培的两种粒型6个品种的系统测定,NRA的平均值亦与其耐肥性呈负相关,与花生叶片NO_3^-—N含量和籽粒蛋白质含量呈正相关,所以,花生NRA的高低可作为生化育种的指标.在不同氮肥量做种肥的条件下,种肥用量越高,苗期NO_3^-—N含量越高,NRA越低,氮肥抑制了苗期NRA.

The work showed that the highest nitrate reductase activity (NRA) appe-aedr in the functional period of each leaf on main stem of groundnut, which could last for a period(6days) and then dropped. In the whole growing period of groundnut, an obvious peak of NRA came out in seedling stage. In seedling stage NRA was inversely correlative with nitrogen response of varieties. Through measuring and assaying 6 varieties grown in field successively , the results showed that the mean value of NRA was inversely correlative with the nitrogen response of varieties, but directly correlative with the amount of NO-3-N in leaves and protein in seeds.So it was considered that NRA of groundnut could be used as an index in its biochemical breeding. In the treatment of N fertilizer as seed manure, the more N fertilizer was applied, the higher the amount of NO-3-N in seedling ,stage, the lower the NRA.So it was concluded that N fertilizer could inhibit the NRA of seedling stage.