流动剪切力作用下原代大鼠成骨样细胞PGE_2的表达

Effects of Fluid Shear Stress on PGE-2 Synthesis of Primary Osteoblast-like Cells in Rats

目的 :探讨流动剪切力调节成骨细胞增殖和分化的信号转导过程中PGE2 的作用机制。方法 :对体外培养的大鼠成骨样细胞施加 0 12mN cm2 的流动剪切力 ,采用放射免疫法检测细胞受力 5、10、15、30、6 0、12 0min后不同时段的PGE2 的表达。结果 :大鼠成骨样细胞受到 0 12mN cm2 流动剪切力作用后PGE2 的生成与空白对照组相比明显提高 (P <0 0 1) ,PGE2 的生成在受力后 10min开始显著增加 (P <0 0 1) ,6 0min后达到高峰 (P <0 0 1)。结论 :PGE2 途径是将流动剪切力刺激信号转导进入成骨细胞 ,促进骨改建的信号转导途径之一。

Objective:The purpose of this study is to study the function of PGE-2 during the signal transduction in which fluid shear stress regulates the proliferation and differentiation of osteoblast_like cells.Methods: The isolated rat primary osteoblast_like cells were exposed to fluid shear stress 0 12 mN/cm+2 for 5, 10, 15, 30, 60 and 120 minutes respectively in a flow chamber. The release of PGE-2 was examined. Results: After exposure to fluid shear stress, the PGE-2 synthesis of rat primary osteoblast-like cells increased significantly (P<0 01) when compared with the control. After 10 minutes, the release of PGE-2 began to increase significantly (P<0 01) and the effect was maximal after 60 minutes (P<0 01).Conclusion: PGE-2 pathway may be one of the signal_transduction pathways which can transduce the fluid shear stress into osteoblast_like cells and then stimulate the bone remodeling.