新生儿缺氧缺血性脑病与NO、NOS、PRL水平的相关性研究

Correlated study for the levels of NO,NOS and PRL with neonatal hypoxic-ischemia encephalopathy

采用放免法和比色法测定68例新生儿缺氧缺血性脑病(HIE)及30例正常新生儿血浆一氧化氮(N0)、一氧化氮合酶(NOS)及血清催乳素(PRL)活性水平,以探讨NO、NOS及PRL在HIE中的发病机理、病情进展及预后中的作用。结果;HIE急性期NO、NOS、PRL水平较正常新生儿明显升高(P<0.05),中度和重度HIE的3项水平升高更显著(P均<0.01);HIE惊厥组急性期血清PRL水平显著高于非惊厥组(P<0.01),且与惊厥发作持续时间呈正相关(r=0.485,P<0.01)。提示:NO、NOS、PRL水平高低与HIE患儿脑损伤严重程度有关,可作为判断病情、预后及惊厥是否发作的指标。

To explore the role of nitric oxide(NO). nitric oxide synthetase (NOS)and prolactin (PRL) in the patho-genesis,progress and prognosis of neonatal hypoxic-ischemia encephalopathy (HIE),plasma NO, NOS and serum PRL were determined by radioimmunoassay(RIA) and colorimetry in 68 newborn infants with HIE and 30 normal control neonates, respectively. The results showed that the levels of NO, NOS and PRL were significantly higher in all neonates with HIE than that in the control group (P<0. 05). There were in particular more higher levels of NO,NOS and PRL in infants with either moderate or severe HIE(P<0. 01). It was noticed that the level of PRL was much higher in HIE infants with seizures than that in HIE infants without seizures. There was a positive relationship between the levels of PRL and the duration of seizure attack(P<0. 01). It is suggested that the levels of NO, NOS and PRL is associated with HIE. The determination of these parameters will be valuable clinically for the diagnosis, judgement of prognosis and estimation of seizure attack in newborn infants with HIE.