糖尿病大鼠睾丸病理变化及脂质过氧化和一氧化氮的改变

Study on testicular pathological change, lipid peroxidation and nitric oxide in diabetic rats

目的 :研究糖尿病睾丸的病理变化及其发生机制。方法 :用光镜及透射电镜观察四氧嘧啶诱导的糖尿病 1个月大鼠睾丸的形态学改变 ,并测定睾丸组织超氧化物歧化酶 (SOD)、谷胱甘肽过氧化物酶 (GSH -PX)、一氧化氮合酶 (NOS)的活性及一氧化氮 (NO)、丙二醛 (MDA)含量。结果 :光镜下主要表现为睾丸曲细精管萎缩及生精阻滞 ;透射电镜下主要见到支持细胞线粒体与内质网扩张、胞浆内内含物形成 ;睾丸组织的SOD、GSH -PX活性实验组低于对照组 ,NOS活性及NO、MDA含量实验组高于对照组。结论 :糖尿病大鼠睾丸病变主要为支持细胞受损及生精障碍 。

AIM: To study testicular pathological change and its pathogenesis. METHODS:Testicular structure of alloxan induced diabetic rats was observed under light microscopy (LM)and transmission electron microscopy(TEM). Activity of superoxide dismutase(SOD),glutathione peroxidase(GSH-PX),nitric oxide synthase(NOS)and content of malondialdehyde (MDA),nitric oxide(NO) were detected biochemically in testicular homogenate. RESULTS: It is manifestated as atrophy of seminiferous tubule and germinal arrest under LM and expansion of mitochondria and smooth endoplasmic reticulum, cytoplasmic inclusion of sertoli cell under TEM.Activity of SOD, GSH-PX decreased while activity of NOS, content of MDA, NO increased in diabetic rats compared with control one. CONCLUSION: Disturbance of spermatogenesis and damage of sertoli cell are the main morphological change of diabetic testis, lipid peroxidation and NO may be involved in it.