CD44v3、CD44v6在非小细胞肺癌中的表达及其与淋巴结转移的关系

Expression of CD44v3 and CD44v6 in patients with non small cell lung cancer and its relationship with lymph node metastasis

目的 研究CD44v3、CD44v6在肺癌中的表达及其与肺癌淋巴结转移的关系。方法 采用免疫组化、流式细胞术测定5 2例非小细胞肺癌 (NSCLC)和 1 2例正常肺组织的CD44v3、CD44v6的表达水平 ,并分析其与肺癌淋巴结转移之间的关系。结果 肺癌组织CD44v3、CD44v6水平明显高于正常肺组织 (P <0 .0 5 )。CD44v3、CD44v6在淋巴结转移组中的阳性表达率显著高于无淋巴结转移组 (P <0 .0 5 )。结论 CD44v3、CD44v6在非小细胞肺癌中有不同程度的表达 ,CD44v6与肺癌淋巴结转移的关系更密切 ,表达CD44v6的肺癌更易向周围淋巴结转移。检测CD44v6在肺癌中的表达 ,可能作为判断肺癌淋巴结转移潜能的一个辅助指标。

objective To study the expression of CD44v3 and CD44v6 and its relationship with lymph node metastasis in non small cell lung cancer (NSCLC). Methods Specimens (lung tissues) from 52 cases of NSCLS and 12 normal lung tissue were used to detect the expression of CD44v3 and CD44v6 by immunohistochemical method (SP method) and flow cytometry, correlation was analysed between the expression of CD44v3 or CD44v6 and lymph node metastasis of the lung cancer. Results CD44v3 and CD44v6 were not, or weakly expressed in all normal lung tissues from 12 cases. In contrast, the expression levels of CD44v3 and CD44v6 were obviously higher in lung cancer than that in normal tissue(P< 0.05) . The expression of CD44v3 and CD44v6 were much higher in patients with lymph node metastasis than those without lymph node metastasis(P<0.05). Conclusion ①CD44v3 and CD44v6 are expressed with different degree in NSCLC. ②There is a close relationship between high expression of CD44v6 and lymph node metastasis, and CD44v6 may be a co marker for predicting the potentiality of lymph node metastasis in lung cancer.