灵芝及其与丹参、五味子、柴胡配伍对小鼠实验性肝损伤的影响

Effects of Ganoderma lucidum and it combined with Radix Salviae Miltiorrhizae, Radix Bupleuri, and Fructus Schisandrae Chinensis respectively on experimental hepatic injuries in mouse

目的 探讨灵芝醇提物分别与柴胡、丹参、五味子醇提物配伍后对小鼠实验性肝损伤的保护作用。方法 分别采用四氯化碳 (CCl4 )或D 氨基半乳糖 (D GlaN)诱导的小鼠肝损伤模型 ,给予同一剂量的药物 ,测定血清及肝组织ChE和ALT活性 ,肝组织MDA、ALP、GSH含量 ,VG染色观察肝组织形态学变化。结果 CCl4 所致小鼠血清ALT急剧升高 ,ChE显著下降 ,灵芝及灵芝 +五味子、灵芝 +柴胡组有显著降低和升高作用 ;灵芝各配伍组可显著抑制GSH的升高 ,对MDA的升高只有灵芝 +五味子可显著抑制 ,使其降至正常水平。对D GlaN所致模型组的ChE活性影响不明显 ;灵芝组可使显著升高的ALT活性明显降低 ;对GSH的升高 ,灵芝 +丹参有影响 ,但差异无显著性。灵芝及灵芝各配伍组对升高的MDA有显著降低作用 (P <0 .0 1) ,病理切片观察表明 ,灵芝及灵芝 +五味子组对肝细胞的损伤有较好的修复作用。结论 灵芝对实验性肝损伤有一定的保护作用 ,与五味子配伍作用更明显。

Objective To investigate the protective effects of alcoholic extracts of Ganoderma lucidum (GL) and its combination with Radix Salviae Miltiorrhizae (RSM), Radix Bupleuri (RB), and Fructus Schisandrae Chinensis (FSC) respectively on experimental hepatic injuries. Methods Hepatic injury models were established with the injections of carbon tetrachloride (CCl 4) or D galactosamine(D GlaN) into mouse, and then the therapeutic agents at same dose were given respectively. The activity of cholinesterase(ChE) and alaline aminotransferase(ALT) in the serum and hepatic homogenate, the contents of malonyl dialdehyde (MDA), alkaline phosphatase (ALP) and reduced glutathione (GSH) in hepatic homogenate from all mouse were determined. The morphologic changes of the livers were also observed with VG staining. Results In CCl 4 treated mice, serum ALT activity was increased markedly while ChE activity decreased significantly. GL and GL+RB, GL +FSC could relieve these changes; GL combined with all other agents could inhibit the GSH accumulation, but only GL+FSC had a suppressive effect on MDA increase and made it return to normal. For D GlaN treated mice, all agent groups had little effect on the activity of ChE; Only GL dramatically reduced the increased activity of ALT; GL +RSM could eliminate the GSH, but not significant; GL and all GL combinations could decrease the increased MDA ( P <0.01). Pathological observation showed that hepatocyte damage in GL group and GL+FSC group inclined to recover. Conclusion The results indicate that Ganoderma lucidum is an effective agent against hepatic injuries, especially combined with Fructus Schisandrae Chinensis, which may be associated with its anti hepatocyte oxidation.