L-丙氨酰-L-谷氨酰胺的合成及初步鉴定

Synthesis and preliminary identification of L-alanyl-L-glutamine dipeptide

目的 探讨L 丙氨酰 L 谷氨酰胺双肽的化学合成方法。方法 以L 谷氨酰胺和 2 D 氯丙酰氯为原料 ,通过缩合、胺化等反应 ,合成L 丙氨酰 L 谷氨酰胺双肽 ;采用熔点、氨基酸组分分析、元素分析、质谱等方法对其结构进行鉴别 ,用高效液相色谱法确定其纯度。结果 合成的双肽经理化检测 ,质量高。结论 采用本法合成谷氨酰胺双肽具有简便、快速、质量高等优点。

Objective To explore the method of chemical synthesis of L alanyl L glutamine dipeptide. Methods L alanyl L glutamine dipeptide was prepared by condensation and ammoniation reaction of L glutamine and 2 D chloropropionyl chloride. The physico chemical properties of this dipeptide were identified with melting point determination, amino acid analysis, elements analysis and MS and its purity was determined with HPLC. Results This dipeptide was confirmed good in quality by physico chemical determination. Conclusion Dipeptide synthetized by this method is simple, convenient and with good guality.