大肠癌及转移癌组织的微血管计数及VEGF表达的意义

Microvessel quantitation and VEGF expression in colorectal carcinoma and metastatic tissues

目的 研究大肠癌与转移癌组织的血管生成情况 ,探讨血管生成与肿瘤转移和预后的关系。方法 应用免疫组化法 ,研究了 15例有淋巴结和肝转移的大肠癌、2 0例无转移的大肠癌和正常结肠粘膜的石蜡包埋组织的微血管计数 (MVD值 )和血管内皮生长因子 (VEGF)表达。结果 大肠癌和转移癌组织的MVD值明显高于正常粘膜 ,发生转移的大肠癌组织的MVD值明显高于未发生转移的大肠癌组织 ;发生转移的大肠癌及其转移癌组织中VEGF阳性组值明显高于VEGF阴性组。

Objective To explore the relationship between angiogenesis and tumor metastasis and prognosis by studying the angiogenesis in colorectal carcinoma tissues and metastasized tissues. Methods The microvessel density (MVD) and the expression of vascular endothelial growth factor (VEGF) were studied with immunohistochemical assays in 15 samples of colorectal carcinoma with lymph node and liver metastasis, 20 specimens of colorectal carcinoma without metastasis. Normal rectal mocosal tissues were taken from 10 cases of colorectal carcinoma with metastasis and 10 without. Results The MVD was obviously higher in the cases of colorectal carcinoma with or without metastasis than in normal rectal tissues, and that in those with metastasis was higher than that of those without. The MVD was significantly higher in those positive to VEGF staining than those negative in colorectal carcinoma tissues and metastasized tumors. Conclusion The MVD and VEGF expression can be regarded as indexes for tumor metastasis and prognosis in colorectal carcinoma.