鼻咽上皮癌变过程中端粒酶活性和端粒酶RNA的表达

Profile of telomerase and telomerase RNA expression in nasopharyngeal carcinogenesis of rats induced by N, N'dinitrosopiperazine (DNP)

目的 研究二亚硝基哌嗪 (DNP)诱发大鼠鼻咽癌变过程中端粒酶的表达规律。方法 以DNP诱导大鼠鼻咽癌 ;用PCR ELISA和NestedRT PCR检测DNP诱导大鼠鼻咽癌变不同阶段端粒酶活性和端粒酶RNA的表达 ,同时作病理形态学检测。结果 DNP诱导大鼠鼻咽癌变过程中 ,端粒酶活性不断升高 ,端粒酶的变化与鼻咽癌变呈正相关 ,而且端粒酶中RNA表达先于端粒酶的表达。在大鼠鼻咽上皮细胞异型增生阶段即出现端粒酶的激活和端粒酶RNA的表达。结论 化学致癌物DNP诱导细胞癌变端粒酶的激活 ,且端粒酶的激活和端粒酶RNA的表达是鼻咽上皮癌变的早发事件 ,与鼻咽癌的发生、发展有关。

Objective To investigate the profile of telomerase and telomerase RNA expression in nasopharyngeal carcinogenesis (NPC) induced by N,N'dinitrosopiperazine (DNP) and examined histolofically. Methods Nasopharyngeal carcinomas of rats were induced by DNP and examined histologically. PCR ELISA and nested RT PCR were used to assay telomerase and telomerase RNA expression at different stages in the nasopharyngeal tissues of rats. Results During the carcinogenesis process, telomerase activity increased along with the formation of a nasopharyngeal carcinoma. Telomerase expression was positively related with nasopharyngeal carcinogenesis. Telomerase RNA expression was present and did not change during the NPC process. Expression of telomease RNA was earlier than telomerase activation. Telomerase activation and telomerase RNA expressin were also detected in the pre cancerous nasopharyngeal lesions. Conclusion Telomerase activation may participate in the onset and progression of NPC, and is an early step in NPC.