药物性胃粘膜损伤大鼠ICAM-1及TNF-α表达的研究

STUDY ON THE EXPRESSION OF ICAM-1 AND TNF-α IN GASTRIC MUCOSAL CELL DURING DRUG INDUCED GASTRIC MUCOSAL INJURY IN RAT

为了解胃粘膜损伤时胃粘膜细胞有无细胞间粘附分子 1(ICAM 1)及肿瘤坏死因子 (TNF α)的表达 ,采用SD大鼠灌服阿司匹林的方法建立胃粘膜损伤模型 ,用免疫组化法动态观察了用阿司匹林致胃粘膜损伤后大鼠胃粘膜ICAM 1、TNF α的表达。结果显示 ,ICAM 1在用阿司匹林后 30min时开始表达 ,1h达高峰 ,高峰持续到 6h ,12h开始下降 ,2 4h仅有少量表达 ;TNF α在用阿司匹林后 15min开始表达 ,3h达高峰 ,持续到 6h ,12h开始下降 ,2 4h表达仍较明显。对照组及用药前胃粘膜细胞仅有少量ICAM 1及TNF α表达。提示ICAM 1及TNF α的表达在胃粘膜损伤的发病机制中有重要意义。

To investigate the expression of ICAM 1 and TNF α in gastric mucosal cell during drug induced mucosal injury, the authors carried out the study in SD rats with aspirin by mouth with immunohistochemical method . The results showed that ICAM 1 began to express at 30min after administration of aspirin, peaked at 1h, maintained until 6h, decreased at 12h,and expressed slightly at 24h . TNF α began to express at 15min, peaked at 3h to 6h,decreased at 12h, and expressed significantly at 24h. There was a slight ICAM 1 and TNF α expression in gastric mucosal cells in the control group. It was suggested that the expression of ICAM 1 and TNF α plays an important role in the mechanisms of gastric mucosal injury .