人树突状细胞体外提呈与EB病毒感染相关肿瘤抗原的研究

Generation of dendritic cells from human bone marrow and efficient presentation of tumor antigen associated with Epstein-barr virus

目的建立从人骨髓造血前体细胞体外培养扩增树突状细胞的方法 ,制备 EBV感染介导的相关肿瘤 (如何杰金氏病、鼻咽癌和 Burkitt's淋巴瘤等 )的疫苗。方法采用 Mini- MACS分离技术从正常人骨髓分离 CD34+ 造血干 /祖细胞 ,体外以重组细胞因子组合诱导培养 2周 ,同种混合淋巴细胞反应检测扩增 DCs的 T淋巴细胞刺激活性。结果从正常人骨髓分离得到高纯度 (>90 % )的 CD34+造血干 /祖细胞 ,经重组 h GM- CSF、h TNF- α、h IL- 3、h IL- 4的共同诱导培养 ,扩增得到大量DCs。扩增的 DCs表面具有不规则突起 ,高表达共刺激分子 CD1α,对同种异体 T淋巴细胞具有很强的刺激活性 ,负载抗原后刺激活性进一步增强。结论人 CD34+ 造血干 /祖细胞体外经 h GM- CSF、h TNF-α、h IL - 4、h IL - 3共同诱导培养 ,可生成大量功能成熟的 DCs,并可有效提呈与 EBV感染相关肿瘤的抗原 ,可望用于 EBV感染介导相关肿瘤的疫苗治疗。

ObjectiveTo expand dendritic cells (DCs)from human bone marrow and design vaccine against cancer concerned with the infection of EBV,for instance,Burkitt's lymphoma、nasopharyngeal cancer、Hodgkin's disease and so on in vitro . MethodsCD34 + hematopoietic stem cells were isolated from normal bone marrow by mini-MACS.Then CD34 + hematopoietic stem cells were cultured in hGM-CSF、hTNF-α、hIL-3、hIL-4 for two weeks. T cells stimulating activity was determined by allo-MLR. ResultsThese cells expressing high levels of CD 1α have typical dendritic morphology. They could acquire Epstein-Barr virus latent membrane glycoprotein gp340 efficiently and induce T cells increasing stimulatory capacity in MLR. ConclusionWe could obtain large amount of mature DCs by in vitro culture of human CD34 + hematopoietic stem cells. It will probably become a potent approach of gp340-loaded DC against tumor in relation to EBV. [