摘要
目的 研究体外培养及转移生长因子 - β(TGF - β) )对骨髓基质细胞生长的影响。 方法 抽取兔骨髓基质细胞体外培养 ,倒置显微镜动态观察 ,并在培养液中加入TGF - β。 结果 从原代培养细胞 (P0 )中观察到细胞集落的形态有明显差异 ,提示骨髓基质细胞是一个由多种细胞组成的混合体。经TGF - β处理的细胞 ,生长速度明显增快 ,传 30代以上仍能保持较活跃的增殖能力。而未经TGF - β处理的细胞 ,活跃增殖能力约只能保持 10～ 15代 ,随后即发生退化。结论 TGF - β能促进骨髓基质细胞增殖并较长期保持其生物活性。
Objective To study the morphologic and phenotypic effects of transforming growth factor-beta(TGF-β) on cultured marrow stromal cells(MSC) in vitro. Methods The MSC was cultured in vitro supplemented with or without TGF-β at a concentration of 5 ng/ml, and the cell morphology and the speed of cell growth were observed under inverted microscope.Results Without the treatment of TGF-β,the MSC degenerated after 10～15 passages; While treated with TGF-β, the increased cellular density with cell layering and nodule formation were well observed ,and the cells kept their bioactivity for more than 30 passages.Conclusion Treated with TGF-β facilitates the proliferation of MSC, and keeps their bioactivity for quite long time.
出处
《苏州医学院学报》
2001年第2期129-130,148,共3页
Acta Academiae Medicinae Suzhou
