运用ELISA快速检测CPV抗体方法的建立与均衡性的研究 I.间接ELISA快速检测CPV抗体方法的建立

I. Studies on Development of an Indirect ELISA for Detecting Antibody to Canine Parvovirus

用蔗糖密度梯度离心和凝胶层析纯化犬细小病毒(CPV)作抗原，建立了检测CPV抗体的间接酶联免疫吸附试验(ELISA)，其特异性和稳定性良好，结果判定精确明显，易于把握。

An indirect Enzyme－linked immunosorbent assay(ELISA)for the detection of antibody to Canine Parvovirus(CPV)in dogs had been developed with viral antigen purified by sucrose density gradient centrifugation and gel chromagraph sepherose2B． The performance of ELISA was assessed for reproducibility and specificity．The results showed that the ELISA had good reliability and accessibility．