牛卵形巴贝斯虫的体外培养

THE CULTIVATION OF BABESIA OVATA IN VITRO

本试验研究了长期在液氮或 - 80°C冷冻保存虫株 -牛卵形巴贝斯虫 (B.ovata)的体外培养方法 ,并探讨了培养条件。试验结果表明 :液氮内保存 2年之久的牛卵形巴贝斯虫 980 814号虫株 ,先经过 BO- SCID小鼠体内大量增殖后 ,在完全培养液和 37°C,5 % CO2 ,5 % O2 ,90 % N2 培养箱内能够快速增殖 ,连续培养 45天 ,继代 9次。B.ovata在 BO- RBC- SCID小鼠体内染虫率可达 15 %以上 ,体外培养最高染虫率为 6 .3%。染虫率 5 %以上红细胞可作诊断用抗原的制备材料或用液氮 (或冷冻 )继续保存。

The cultivation of B.ovata in vitro, which had been kept frozenly in the liquid nitrogen for 2 years was studied in present experiment. The results showed that B.ovata 980814 could develop very fast in the condition when temperature was 37 °C , microaerophilic nature 5% CO 2, 5% O 2, 90% N 2, after developing in the BO RBC SCID mouse by inculation. Furthermore, the B.ovata 980814 could be cultivated continuously for 45 days, and subcultured for 9 times. The percentage of parasitied erythrocyted (PPE) in the BO RB SCID mouse was 15% (24h), and maxium parasitemias was 6.3% in vitro. The results indicated that PPE higher than 5% could be used for the diagnosis and can be served further in the liquid nitrogen or in the frozen condition.