乙烯受体基因LeETR1在番茄Epi和VFN8中的表达及反义表达载体构建(英文)

Expression of ethylene receptor gene LeETR2 in tomato mutant Epi and construction of its antisense expression vector

采用 RPA方法对番茄乙烯过表达单基因突变体 Epi和野生型 VFN8中 L e ETR2 m RNA的表达特征进行了研究 .结果表明 ,在所有被检组织中 Le ETR2 m RNA均表达 ,其表达丰度在叶组织中呈发育调节模式 ,但不受内源乙烯含量的影响 ;而在果实成熟后期受乙烯的轻微诱导 . Le ETR2的这种表达模式明显有别于其他乙烯受体基因 .为了进一步研究 Le ETR2的功能 ,构建了 Le

The expressive characteristics of LeETR2 mRNA in tomato mutant Epi and its wild type VFN8 have been studied with RNase protect analysis (RPA) technique. The results showed that LeETR2 mRNA expressed in all the tissues examined, and it's expression was unaffected by the endogenous ethylene in leaves. However, LeETE2 mRNA was developmentally regulated, and partially induced by ethylene in fruits. The results indicated that LeETR2 mRNA exhibits different expression pattern from other ethylene receptor genes in tomato. Moreover, LeETR2 antisense gene expression vector has been constructed for further research.